Identification and Expression of Nine Oak Aquaporin Genes in the Primary Root Axis of Two Oak Species, Quercus petraea and Quercus robur
نویسندگان
چکیده
Aquaporins (AQPs) belong to the Major Intrinsic Protein family that conducts water and other small solutes across biological membranes. This study aimed to identify and characterize AQP genes in the primary root axis of two oak species, Quercus petraea and Quercus robur. Nine putative AQP genes were cloned, and their expression was profiled in different developmental root zones by real-time PCR. A detailed examination of the predicted amino acid sequences and subsequent phylogenetic analysis showed that the isolated AQPs could be divided into two subfamilies, which included six plasma membrane intrinsic proteins (PIPs) and three tonoplast intrinsic proteins (TIPs). We characterized the anatomical features of the roots and defined three developmental root zones: the immature, transition and mature zones. Expression analysis of the AQPs was performed according to these root developmental stages. Our results showed that the expression of PIP2;3 and TIP1 was significantly higher in Quercus petraea compared with Quercus robur in the three root zones. However, PIP2;1 and TIP2;1 were found to be differentially expressed in the mature zone of the two oak species. Of the nine AQP genes identified and analyzed, we highlighted four genes that might facilitate a deeper understanding of how these two closely related tree species adapted to different environments.
منابع مشابه
Comparative Pollen Morphological Analysis and Its Systematic Implications on Three European Oak (Quercus L., Fagaceae) Species and Their Spontaneous Hybrids
Pollen morphology of three parental Quercus species (Q. robur L., Q. petraea (Matt) Liebl, Q. pubescens Willd.) and two spontaneous hybrids of these species (Q. ×calvescens Vuk. = Q. petraea × Q. pubescens and Q. ×rosacea Bechst. = Q. robur × Q. petraea) was investigated in this study. The pollen originated from 18 natural oak sites and 67 individuals (oak trees). Each individual was represente...
متن کاملIntra- and interspecific diversity in the response to waterlogging of two co-occurring white oak species (Quercus robur and Q. petraea).
Quercus robur L. and Quercus petraea (Matt.) Liebl. are sympatric oak species with different ecological requirements. Quercus robur is more tolerant to waterlogging than Q. petraea. This ecological divergence may play a role in the maintenance of the two species despite the absence of an insurmountable reproductive barrier between them. We predicted that the genetic architecture of traits relat...
متن کاملResponses of two closely related oak species, Quercus robur and Q. petraea, to excess manganese concentrations in the rooting medium.
Quercus robur (L.) and Q. petraea (Matt.) Liebl. are European oak species that often grow in forest soils with high soluble manganese (Mn2+) concentrations. We tested the effects of Mn2+ at concentrations of 0.0024 mM (control), 0.24 mM (typical of acidic forest soils) and 1.2 mM (typical of forest soils under strongly reducing conditions) on the growth, tissue anatomy, foliar element concentra...
متن کاملDistinct male reproductive strategies in two closely related oak species.
Reproductive strategies of closely related species distributed along successional gradients should differ as a consequence of the trade-off between competition and colonization abilities. We compared male reproductive strategies of Quercus robur and Q. petraea, two partly interfertile European oak species with different successional status. In the studied even-aged stand, trees of the late-succ...
متن کاملEnvironmental conditions and oak barrels timber quality: Which is the influence in the Quercus petraea Matts. Liebl. forests in the Iberian Peninsula?
The autoecology of the oak Quercus petraea in the northwest Iberian Peninsula was investigated in the present study by applying the methodology developed in previous studies of other species of the genus Quercus. For this, the distribution of the species was firstly determined so that a minimum number of representative species could be selected. A total of 52 plots were chosen and in each one, ...
متن کامل